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KMID : 1007020100080020178
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Korean Soceity of Osteroporosis
2010 Volume.8 No. 2 p.178 ~ p.187
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The Relationship between Wnt Signaling Pathway Gene Polymorphisms and Production of Osteoprotegerin and Soluble Receptor Activator of NF-kB by Whole Blood Cells
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Lee Dong-Yun
Suh Chang-Suk
Ku Seung-Yup
Kim Jung-Gu
Kim Seok-Hyun
Choi Young-Min
Kim Hoon
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Abstract
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Objectives: To investigate the relationship between single nucleotide polymorphisms (SNPs) in Wnt signal pathway genes, and production of osteoprotegerin (OPG) and soluble receptor activator of NF-κB ligand (sRANKL) by whole blood cells in postmenopausal Korean women.
Methods: Low density lipoprotein receptor-related protein (LRP) 5 c266 A>G and c3893 C>T, frizzled (FZD) 6 c1033 A>C, axin II c148 C>T, T cell factor (Tcf) 1 c766 G>A, and adenomatous polyposis coli (APC) c5465 T>A polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), direct sequencing, and Taqman assay in 141 postmenopausal Korean women. The production of OPG and sRANKL by lipopolysaccharide-stimulated whole blood cells were also measured.
Results: The production of OPG and sRANKL by lipopolysaccharide-stimulated whole blood cells, and their ratio of RANKLx1,000/OPG were not different according to the LRP 5 c266 A>G and c3893 C>T, FZD 6 c1033 A>C, axin II c148 C>T, Tcf 1 c766 G>A, and APC c5465 T>A polymorphisms. No significant differences in the production of OPG and sRANKL, and their ratio of RANKLx1,000/OPG were noted among combined genotypes of LRP c266 A>G and c3893 C>T polymorphisms.
Conclusion: LRP 5 c266 A>G and c3893 C>T, FZD 6 c1033 A>C, axin II c148 C>T, Tcf 1 c766 G>A, and APC c5465 T>A polymorphisms do not affect the production of OPG and sRANKL by lipopolysaccharide- stimulated whole blood cells.
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KEYWORD
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Whole blood cell, Osteoprotegerin, RANKL, Polymorphism
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